A PCR-High-Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus
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چکیده
منابع مشابه
A PCR high - resolution melt for the rapid differentiation of nontypable Haemophilus influenzae 1 and Haemophilus haemolyticus
A PCR high-resolution melt for the rapid differentiation of nontypable Haemophilus influenzae 1 and Haemophilus haemolyticus 2 3 Janessa Pickering, a # Michael J Binks, b Jemima Beissbarth, b Kim M Hare b , Lea-Ann S 4 Kirkham, a,c Heidi Smith-Vaughan. b 5 6 School of Paediatrics and Child Health, The University of Western Australia, Perth, Australia, a 7 Child Health Division, Menzies School o...
متن کاملRelationships of nontypeable Haemophilus influenzae strains to hemolytic and nonhemolytic Haemophilus haemolyticus strains.
Haemophilus influenzae is both a human respiratory pathogen and pharyngeal commensal, while H. haemolyticus, the closest phylogenetic relative of H. influenzae, is arguably a strict pharyngeal commensal. A hemolytic phenotype has historically differentiated H. haemolyticus from H. influenzae, but the recent recognition of significant nonhemolytic H. haemolyticus colonization has decreased this ...
متن کاملVaccine Candidates against Nontypeable Haemophilus influenzae: a Review
Nonencapsulated, nontypeable Hemophilus influenzae (NTHi) remains an important cause of acute otitis and respiratory diseases in children and adults. NTHi bacteria are one of the major causes of respiratory tract infections, including acute otitis media, cystic fibrosis, and community-acquired pneumonia among children, especially in developing countries. The bacteria can also cause chronic dise...
متن کاملPCR screening for the N526K substitution in isolates of Haemophilus influenzae and Haemophilus haemolyticus.
OBJECTIVES Firstly, to evaluate the current PBP3-S primers of Hasegawa et al. (Microb Drug Resist 2003; 9: 39-46) and develop new primers for the amplification of N526 in isolates of Haemophilus haemolyticus. Secondly, to develop a new PCR assay for the detection (by amplification) of the N526K substitution, encoded by either the AAA or AAG single nucleotide polymorphism (SNP) at position 1576-...
متن کاملComparative Genomic Analysis of Haemophilus haemolyticus and Nontypeable Haemophilus influenzae and a New Testing Scheme for Their Discrimination.
Haemophilus haemolyticus has been recently discovered to have the potential to cause invasive disease. It is closely related to nontypeable Haemophilus influenzae (NT H. influenzae). NT H. influenzae and H. haemolyticus are often misidentified because none of the existing tests targeting the known phenotypes of H. haemolyticus are able to specifically identify H. haemolyticus Through comparativ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2013
ISSN: 0095-1137
DOI: 10.1128/jcm.02191-13